안녕하십니까?

저는 동아대학교의료원 비뇨기과학 교실 전공의 2년차 신찬욱이라고 합니다.

다름이 아니라.. 이번에 저희 교수님께서 논문을 준비 하시면서

준비 내용중에 Microarray 라는 방법을 사용해서 gene expression을 보는

내용이 있던데..

이 방법이 가능한지 한번 알아 보라고 하셔서 문의차 mail드립니다.

(저희 병원 병리과에 문의 해 보니 자연과학부에서 gene expression을 보는 기계를

구입 했다고 하면서 자연

제가 전공자가 아니라 논문을 봐도 자세한 내용은 잘 모르겠어서.

논문 초록을 아래에 첨부 하겠습니다.

혹시 이러한 방법으로 실험 가능한지 답변 주시면 감사하겠습니다.

(Cryptorchidism이란 정류고환이라는 질환으로 고환이 음낭 속으로 내려

오지 않고 복부나 사타구니에 머무르는 질환인데 불임이나 종양 가능성

때문에 비뇨기과적으로 수술적 교정을 해 줍니다. 이러한 수술을 받은 환

자들에서 임신 가능성을 어떻게 보는 방법 같은데.. 실험방법을 잘 모르

겠네요..))
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Fertility Potential in the Cryptorchid Male Using Spermatozoal Gene Expression Microarray Analysis

Introduction and Objective: Although previously thought to be transcriptionally inactive, recent studies suggest spermatozoal RNA as a measure of past events during spermatogenesis. Using microarray analysis, we sought to identify significant differential spermatozoal gene expression patterns between adolescent males with a prior history of corrected cryptorchidism and normal control patients. Methods: Ejaculates were collected and a semen analysis performed on 10 control patients (normal urogenital history/exam and semen analysis by WHO criteria) and 12 cryptorchid males (8 unilateral, 4 bilateral). Motile sperm were separated from round cells and nonmotile sperm by two rounds of Percoll centrifugation, the total RNA extracted and verified using sperm-specific RT-PCR. Biotin-labeled amplified RNA was hybridized to Affymetrix Human Genome Focus Arrays, according to the Affymetrix protocol. To identify genes that are differentially expressed between sperm from cryptorchid and control patients, permutation t test was peformed. Results: Median semen volume was not significantly different between control and cryptorchid patients (2.1 vs. 3.0 cc, p=0.56). Median sperm density was markedly decreased in the cryptorchid group (94 vs. 10 million/cc, p=0.06). From the microarray expression data, we identified 47 genes differentially expressed (permutation p < 0.05) between the two groups. Of these, 42 genes were under-expressed in the cryptorchid samples including many transcriptional factors. The expression for Tpx-1, a testis-specific spermatogenic-Sertoli cell adhesion gene, was significantly decreased in the cryptorchid boys. An apoptotic gene TNFAIP3 (TNF-alpha induced protein 3) was highly over-expressed in the cryptorchid samples relative to normal controls. Conclusions: Using microarray analysis we have identified a set of genes differentially expressed between normal controls and males with a history of cryptorchidism. The under-expression of transcriptional genes in the cryptorchid samples may be the cause of poor seminal parameters in boys with a history of cryptorchidism.